| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Walls, Annie E (14)
|
MAPAAC |
5:34.63 |
5:29.61 |
-5.02 |
| 2 |
Lopez-Ona, Marissa A (14)
|
MAPCAC |
5:31.81 |
5:31.21 |
-0.60 |
| 3 |
Lopez-Ona, Gabriella A (14)
|
MAPCAC |
5:31.15 |
5:33.20 |
2.05 |
| 4 |
Fritz, Madison L (13)
|
MANPAC |
5:45.94 |
5:39.44 |
-6.50 |
| 5 |
Peshenko, Anna I (13)
|
MADCAA |
5:43.90 |
5:43.35 |
-0.55 |
| 6 |
Fresa, Noel J (14)
|
MAUPY |
|
5:55.04 |
- |
| 7 |
Delaney, Julia J (14)
|
MAPFY |
6:05.64 |
5:55.90 |
-9.74 |
| 8 |
Williams, Ainsley C (14)
|
MAUN |
5:53.60 |
5:57.20 |
3.60 |
| 9 |
Bubser, Kelci L (13)
|
MAPAAC |
6:01.58 |
5:58.20 |
-3.38 |
| 10 |
Sanders, Mary E (13)
|
MADCAA |
6:15.78 |
6:03.47 |
-12.31 |
| 11 |
Park, Amory S (14)
|
MAFSS |
6:19.05 |
6:10.99 |
-8.06 |
| 12 |
Svetz, Claire V (13)
|
MAPAAC |
|
6:18.40 |
- |
| 13 |
Reifsnyder, Jillian E (14)
|
MANRG |
|
6:47.24 |
- |